Journal: Journal of Virology

Article Title: Immunogenicity of NSDV GP38 and the role of furin in GP38 proteolytic processing

doi: 10.1128/jvi.00537-25

Figure Lengend Snippet: Production and characterization of recombinant NSDV GP38-his/FLAG. ( A ) Alignment of partial GPC sequences from different NSDV strains (Ganjam virus IG 619 = NSDV_India; NSDV 708 = NSDV_Kenya; NSDV H. longicornis China = NSDV_China) and CCHFV (IbAr10200). Numbering corresponds to NSDV_India GPC. Amino acid sequences are displayed starting from their respective N-terminus. For CCHFV, the furin protease cleavage site (RSKR) and SKI-1/S1P cleavage site (RRLL) are underlined in blue. For NSDV, arginine-containing motifs reported to be conserved across the GPCs of different orthonairoviruses are highlighted in orange. Two potential N-glycosylation sites (Asn 220 and Asn 400 ) are highlighted with an orange star. ( B ) For protein purification, the partial NSDV_India GPC sequence (aa 138–445; NSDV GP38-his/FLAG protein) was fused to a C-terminal 6×His- and FLAG-tag both highlighted with an orange underline. ( C ) SDS-PAGE of recombinant NSDV GP38-his/FLAG his-tag purified from Sf9 cells followed by Coomassie blue staining and immunoblot analysis using anti-FLAG primary and horseradish peroxidase-conjugated secondary antibodies.

Article Snippet: NSDV Gn ( NAC-REC31904-500 ) and NSDV Gc ( NAC-REC31906-500 ) from The Native Antigen Company were diluted in 0.01 M PBS and coated overnight on Maxisorp 96-well plates at a concentration of 100 ng/well.

Techniques: Recombinant, Virus, Glycoproteomics, Protein Purification, Sequencing, FLAG-tag, SDS Page, Purification, Staining, Western Blot

Journal: Frontiers in Immunology

Article Title: Serological cross-reactivity between Crimean-Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C

doi: 10.3389/fimmu.2024.1423474

Figure Lengend Snippet: Orthonairovirus test antigens employed during experiments.

Article Snippet: NSDV Gc , ACH99800 (708, Kenya) , HEK293 (Human) , The Native Antigen Company, UK #REC31906.

Techniques: Expressing, Plasmid Preparation

Journal: medRxiv

Article Title: A method to reduce ELISA serial dilution assay workload applied to SARS-CoV-2 and seasonal HCoVs

doi: 10.1101/2021.09.13.21263523

Figure Lengend Snippet: ELISA OD values for 1:40 diluted serum against HCoV S1S2 and N proteins. Black lines are the posterior distribution of differences in group means inferred from a linear model using Student-T distributed error. The upper and lower boundaries and mean of the 95% highest posterior density interval of difference in group means is shown in the top right.

Article Snippet: Other antigens were purchased from Sino Biological (OC43 S1S2, catalog number 40607-V08B; HKU1 S1S2, 40606-V08B; NL63 N, 40641-V07E; 229E N 40640-V07E; SARS-CoV-2 N 40588-V08B), BPS Bioscience (NL63 S1S2, 100788), Creative Diagnostics (OC43 N, DAGB101), The Native Antigen Company (229E S1S2, REC31880-100) and Aviva Systems Biology (HKU1 N, OPCA203310).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Viruses

Article Title: Reduced Binding between Omicron B.1.1.529 and the Human ACE2 Receptor in a Surrogate Virus Neutralization Test for SARS-CoV-2

doi: 10.3390/v15061280

Figure Lengend Snippet: Information about the antigens used in the surrogate virus neutralization test for SARS-CoV-2.

Article Snippet: Omicron S1 (B.1.1.529) , Native Antigen , REC32006 , HEK293 , >95% , 16–671 , 29 , 15 f.

Techniques: Neutralization, Expressing